The study demonstrates that a uniquely processed umbilical cord blood product (UCBp) supports proliferation and migration of mesenchymal stem cells (MSCs) and stimulates secretion of factors that promote osteogenic differentiation and neovascularization. These processes are crucial for bone regeneration, suggesting that processed allografts from UCB could have applications in orthopedic treatments like spinal fusions and bone nonunion.
UCBp from 33 donors were profiled in this study to identify and quantify 44 different cytokines, which account for the pro-proliferative, pro-migratory, pro-angiogenic and pro-secretory potential of the UCBp. UCBp induced secretion of VEGF-A by MSCs to enhance angiogenesis in endothelial cells, which illustrates regulation of cellular cross-talk by UCBp. Similarly, UCBp impacted osteogenic differentiation of MSCs by upregulating the secretion of osteoprotegerin.
Bone repair can be a challenging procedure, especially in patients with compromised health. Intensive research using MSC-based cytotherapy has shown promise in orthopedic complications. Cytokines secreted by the MSCs are most likely the primary players that orchestrate cellular functions of the residing host cells to partake in tissue regeneration. The study not only highlights the critical role of cytokines in UCB, but also underpins the importance of processing technique used to preserve its unique molecular heterogeneity in the final clinical product for a positive regenerative outcome.
Read their Open Access article to find out more.
Mustafi SB. Cytokines in umbilical cord blood-derived cellular product: a mechanistic insight into bone repair. Regen. Med. (2018)(ePub ahead of print)