Suspension Culture Medium for human pluripotent stem cells (hPSCs)

Suspension Culture Medium for human pluripotent stem cells (hPSCs)

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Introducing mTeSR™3D, formulated for hPSC expansion in suspension culture.

With its suspension-optimized formulation and simplified fed-batch workflow, mTeSR™3D enables scale up to 1 x 109 undifferentiated hPSCs in as little as 2-3 weeks, starting from a single 6-well plate.

mTeSR™3D and Fed-Batch Culture

mTeSR™3D employs a fed-batch strategy, which eliminates the need for daily medium exchanges, saving you time and medium.

Weekend-free passaging

To save your weekends, the protocol has been optimized with an alternating 3-day/4-day passaging schedule.

Maintenance of hPSC markers

Human PSCs expanded in the mTeSR™3D fed-batch culture system, have robust growth and maintain high expression of hPSC markers.

Look at the experimental data here.

The hPSC aggregates have consistent morphology and are generally uniform in size at the end of each passage and form aggregates of approximately 350-400 μm after 3-4 days in mTeSR™3D.

Browse our website’s technical resources and mTeSR-3D specific page for additional information on scaling up your suspension culture.

Global Exclusive Licensing

mTeSR™3D is manufactured and sold under global exclusive license from Accellta for culture medium for hPSCs in suspension under feeder-free, non-adherent conditions.

Contact STEMCELL Technologies



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STEMCELL™ Technologies

Scientists Helping Scientists™ STEMCELL specializes in developing cell culture media to support basic and translational research which uses pluripotent stem cells and mesenchymal stem cells. In addition to our original line of haematology products, we also offer primary cells and a comprehensive suite of immune cell isolation tools and instruments. Additionally, STEMCELL’s Contract Assay Services department supports researchers by developing tailor-made in vitro assays. Finally, based on our stem cell research expertise, we have grown our product portfolio to include disease modelling tools, media and kits to grow organoids and perform tissue specific differentiation.
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