Regulation of Sequential Release of Growth Factors for Cardiac Tissue Engineering
Bilayer nanoparticles regulate the release of multiple growth factors for cardiac regenerative medicine
Cardiac tissue engineering aims to develop engineered constructs for myocardial infarction repair, where a challenge is the control of growth factor (GF) sequential release. In this study, bilayer polymeric nanoparticles composed of a GF-encapsulating core surrounded by rate-regulating shell have been developed for sequential GF release. Single and bilayer polymeric nanoparticles have been fabricated, characterized and biologically assessed. A novel ‘Geno-Neural model’ has been developed and validated for rate-programming of the nanoparticles. The bilayer nanoparticles feature low burst effect and time-delayed release, and allow for sequential release of PDGF following co-release of VEGF and bFGF, which have shown effective to promote angiogenesis in a cardiac patch hydrogel. The nanoparticulate delivery system, along with the Geno-Neural model, offers great potential for spatiotemporal control of GF release for cardiovascular regenerative medicine.
M. Izadifar, M.E. Kelly, X.B. Chen. NANOMEDICINE Vol. 11, No. 24, Pages 3237-3259 (2016).