Case Study: Automating ELISA-based Assays to Assess the Purity of Therapeutic Viral Vectors

In this Case Study, the Analytical Development group at AGC Biologics (Milan, Italy) describes the automation of two ELISA-based workflows to assess lentiviral vector purity, using a Hamilton Microlab^® STARlet liquid handler integrated with a plate washer, a plate spectrometer, and two plate incubators.  

Highlights of this Case Study: 

• Overview of the analytical methods automated by AGC Biologics Milan on Hamilton liquid Handlers 
• Detailed description of two ELISA-based automated workflows to assess the purity of the lentiviral vectors: residual benzonase and residual Host-Cell Proteins (HCP) 
• Advantages of the Hamilton VENUS Scheduler to plan and execute parallel assays using the same sample aliquot 

23-01-10_Case Study AGC Biologics (1)