Expansion and characterization of human natural killer (NK) cells

Natural killer (NK) cells are an attractive option for immunotherapeutics due to their well-established safety and immunomodulatory characteristics, meaning they could be used ‘off-the-shelf’ avoiding the lengthy expansion and culture associated with autologous treatments. Their function, persistence and targeting could be further enhanced during initial expansion and activation, for example through the insertion of chimeric antigen receptors (CARs). Numerous expansion and activation strategies exist and the most efficient systems, such as engineered cell line feeder-based systems, can achieve over 1000-fold expansion under the right conditions. In this application note, learn how you can optimize your NK cell expansion protocol to achieve an average 1500-fold expansion with this case study featuring feeder-free culture of NK cells grown using the Gibco™ CTS™ NK-Xpander™ Medium.

This content was supplied by Thermo Fisher Scientific.

RMN_THERMO_Expansion-and-characterization-of-human-NK-cells