Creating integration, virus, Myc & Lin28-free induced pluripotent stem cells

Written by Future Science OA

Cellular Engineering Technologies researchers present a new method to generate IPSCs with fewer risks Induced pluripotent stem cells (IPSCs) avoid the ethical controversy of embryonic stem cells (ESCs), but carry neoplastic risk owing to the use of oncogenes c-Myc and Lin28. Work has demonstrated that production can be uncoupled from c-Myc, yet until now an oncogene-free and virus-free method has not been available. New research published in Future Science OA has presented such a method, reprogramming adherent fibroblasts through an episomal expression system. Their combinatorial approach was successful, yielding colonies in which 100% were fully reprogrammed. “Our reprogramming method provides...

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