Cell culture scale-up in stirred-tank single-use bioreactors
Bioprocess development is usually carried out in systems with small working volumes to save time and resources. However, as processes are scaled up, it can become challenging to maintain a constant yield and constant product characteristics. Both product quality and yield can be influenced by gradients in glucose concentration, oxygen and pH. In order to deal with these challenges, development teams must consider multiple factors relating to their bioreactor system, including oxygen supply and culture mixing time.
In this Special Report, Michelet Dorceus (Eppendorf, Hamburg, Germany) discusses key process engineering parameters to ensure the ideal growth environment at all scales, and introduces the BioBLU single-use vessel developed by Eppendorf and its utility in cell culture scale-up.