In Focus: electroporation
Transfection is the process of introducing foreign material – generally nucleic acids (DNA or RNA) – into cells. This allows for the study of gene function and protein expression in the context of the cell, or transformation of the cell into a potential cell therapy product such as a CAR-T. Electroporation is a highly efficient physical transfection method that uses an electrical pulse to create temporary pores in cell membranes for foreign nucleic acids to pass into the cells.
In this In Focus feature, which has been produced in association with Thermo Fisher Scientific (CA, USA), we explore the benefits of optimizing electroporation protocols, as well as the solutions that can be employed to enhance cell therapy workflow.
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