Precision CRISPR editing of induced pluripotent stem cells (iPSCs)

The application of clustered regularly interspaced short palindromic repeats (CRISPR) technology to the field of induced pluripotent stem cells (iPSCs) is showing huge potential in the field of personalized medicine, where the smallest genetic variations are used to predict and address disease outcomes. Genome editing via CRISPR allows highly specific, precise and efficient edits to be made, in order to produce biomimetic disease models, generate relevant cell lines and correct mutations in patient-derived cells. However, iPSCs are challenging to work with in order to maintain pluripotency and unskilled use of CRISPR can lead to high-levels of unwanted off-target effects.

In this Tech Note, learn how to remove roadblocks in your iPSC research using Synthego’s (CA, USA) robust process for producing CRISPR-edited iPSCs and discover their method of producing knockout iPSC pools and clones. Cell pools and clones created using their high-throughput Halo™ Platform come with a guarantee that they will contain your desired knockout, single nucleotide variant or tag.

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