2D to 3D transition of mesenchymal stem cell expansion process using the Sartorius MSC Pilot Solution

Written by Sartorius
App Notes & White Papers Cell therapy iPSCs MSCs Stem cells

In this Application Note, experts from Sartorius (Göttingen, Germany) describe the successful transition of mesenchymal stem sell (MSC) expansion from traditional 2D static culture to 3D microcarrier-based suspension culture using the Sartorius MSC Pilot Solution.

This transition addresses the manufacturing bottlenecks of conventional 2D methods by streamlining the seed train, reducing contamination risks and decreasing labor requirements. Performance comparisons between Univessel® bioreactors and spinner flask controls reveal superior cell growth and viability in the bioreactor systems, establishing a clear pathway for scaling to clinically relevant volumes needed for treating conditions like graft-versus-host disease.

This Application Note is part of the RegMedNet In Focus on iPSCs and MSCs, in association with Sartorius. Discover expert opinions on this topic by visiting our feature homepage.

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This article was provided by Sartorius.