In Focus: Expansion and downstream processing of hiPSCs

Human induced pluripotent stem cells (hiPSCs) hold immense promise for widespread cell therapies, but scaling their production for clinical use is a major obstacle. A novel solution involves microcarrier-based hiPSC 3D cultures in vertical wheel bioreactors, enhancing cell density and enabling precise environmental control.

This In Focus feature delves into the benefits and challenges of microcarrier-based hiPSC scale-up and compares it to conventional 2D-culture-based methods.

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Infographic

Microcarrier-based hiPSC expansion

Explore how adherent cells like hiPSCs can be cultured in suspension by utilizing microcarriers in combination with a vertical-wheel bioreactor. Learn how this system compares to conventional two-dimensional cell cultures when considering scale–up and also how to avoid associated pitfalls.   

 

Interview

Transforming iPSC culture with microcarriers: an interview with Yina Santiago-Rodriguez 

Yina Santiago-Rodriguez gives an overview of microcarrier-based hiPSC scale-up. Yina explains how to overcome challenges by optimizing environmental conditions within the bioreactor to maximize hiPSC differentiation potential. Yina also gives her recommendations for improving the quality control of cell cultures.

Webinar

Scalable expansion and downstream processing of high-quality hiPSCs

This webinar demonstrates why a scalable and reproducible cell expansion process is important for translation from research to clinical manufacturing.

 

 

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