Merging CRISPR editing and single cell sequencing technologies

Written by Teri Arri
3D printing

Keynote address by the Novartis Institutes for Biomedical Reseach

SMi’s conference on 3D Cell Culture will take place on 21st and 22nd February 2018 in London UK and feature a keynote address from Max Salick, Postdoctoral Researcher, Novartis Institutes for Biomedical Research.

MERGING CRISPR-EDITING AND SINGLE CELL SEQUENCING TECHNOLOGIES TO UNCOVER THE BIOLOGY OF HUMAN NEURODEVELOPMENTAL DISEASES IN 3D: CASE STUDIES WITH ZIKA VIRUS AND TUBEROUS SCLEROSIS

Max Salick

Max Salick, Postdoctoral Researcher, Novartis Institutes For Biomedical Research

  • CRISPR-engineered human stem cell lines mimic disease phenotypes during 3D cerebral organoid differentiation
  • Selective removal of target genes in cerebral organoids reveals the biology of Zika virus infection
  • Whole-genome CRISPR screens enable the discovery of new proteins linked to tropism of emerging infectious diseases
  • Single cell sequencing allows researchers to investigate cell-type specific effects of mutations, treatments, or other perturbations in complex 3D tissues

    Further details available at http://www.3d-CellCulture.com/3Dnet