Transitioning your assay from quantitative PCR to droplet digital PCR

Written by RegMedNet


PCR is a powerful technique that can be used to quantify DNA or RNA but is depending on the identity and quality of the standard curve material and operator effects can skew results. Droplet digital PCR (ddPCR) partitions each PCR reaction into individual droplets, physically counting the DNA present and negating the need for a standard curve. In this application note, learn more about migrating assays from PCR to ddPCR for consistency and robustness of results, using the example of an adeno-associated virus (AAV) inverted terminal repeat assay.