Eppendorf 96-Well Cell Culture Plate – A simple method of minimizing the edge effect in cell-based assays

Cell-based assays in multi-well plates are a powerful tool which is widely used in drug discovery, tissue engineering and academic research.

Go to the profile of Eppendorf
Dec 02, 2015

Consistency in the performance of these assays is crucial in order to obtain reliable data and to make valuable scientific statements. In this context evaporation of cell culture medium plays a critical role in multi-well based cellular assays because it can deteriorate assay performance. As evaporation mainly affects the peripheral wells of a plate this phenomenon is called the edge effect. With the Eppendorf 96-Well Cell Culture Plate evaporation from the wells can be reduced to a minimum. The plate offers two options for insulating the wells. A moat surrounding the edge wells and the complete chimney design of the inner wells allow filling of the complete inter-well space with liquid. Together this results in a more homogeneous distribution of temperature and humidity throughout the plate and reduces the edge effect.

Click here to view application note

Interested in learning more? Click here to view an interview with Eppendorf's Dr Daniel Wehrhahn, Director of the Cell Handling Applications, on the challenges in developing cell culture consumables and the importance of observing the cell culture lab environment to create a product that will allow safer, more reproducible research.

Go to the profile of Eppendorf


Life sciences manufacturer, Biotechnology

Eppendorf is a leading life science company that develops and sells instruments, consumables, and services for liquid-, sample-, and cell handling in laboratories worldwide. Its product range includes pipettes and automated pipetting systems, dispensers, centrifuges, mixers, spectrometers, and DNA amplification equipment as well as ultra-low temperature freezers, fermentors, bioreactors, CO2 incubators, shakers, and cell manipulation systems. Consumables such as pipette tips, test tubes, microtiter plates, and single-use bioreactor vessels complement the range of highest-quality premium products. Eppendorf products are most broadly used in academic and commercial research laboratories, e.g., in companies from the pharmaceutical and biotechnological as well as the chemical and food industries. They are also aimed at clinical and environmental analysis laboratories, forensics, and at industrial laboratories performing process analysis, production, and quality assurance. Eppendorf was founded in Hamburg, Germany in 1945 and has more than 3,000 employees worldwide. The company has subsidiaries in 26 countries and is represented in all other markets by distributors. Eppendorf products are widely used within the cell culture workflow. This has been further expanded by the addition of new innovative cell culture consumables and small volume, multi vessel bioprocess systems.

No comments yet.