Systematic bioprocessing: achieve reproducible and robust iPSC expansion and scale-up
Induced pluripotent stem cells (iPSCs) have emerged as a powerful tool for the development and manufacture of advanced therapies. However, their potential is currently limited due to bioprocess challenges in their expansion and scale-up for clinical and manufacturing applications.
In this webinar, we will discuss the available tools and techniques for overcoming these challenges, such as systematic bioprocess design for reproducible and robust iPSC expansion, incorporating a perfusion feeding system for scalable culture of iPSC aggregates and utilization of AOF 3D media to maintain predictable cell growth kinetics, aggregate morphology, and quality outputs.
This webinar was recorded on 13 April 2023.
What will you learn?
- Factors impacting process consistency, such as 2D seed train and harvest protocols, cell quality, hydrodynamic aggregate size control and medium exchange method
- Scalable perfusion culture methods for high yield and quality maintenance of large-scale iPSC production
- The impact of AOF 3D media on the expansion and scale-up of pluripotent stem cell aggregate growth kinetics, morphology, and quality outputs
Who may this interest?
- Clinical researchers developing process workflows for autologous and allogeneic cell and gene therapies
- Those expanding iPSC aggregates for drug screening, disease modeling, or tissue engineering
- Basic, academic, and translational researchers expanding iPSCs or other shear-sensitive cell types
Speaker
Breanna Borys
Bioprocess Scientist
PBS Biotech (CA, USA)
Dr. Breanna Borys is a bioprocess scientist at PBS Biotech (CA, USA). Breanna attended the University of Calgary (Calgary, Canada), where she received her bachelor’s degree in chemical engineering and her PhD in biomedical engineering. In her academic career, Breanna has received numerous prestigious awards, including Engineers Canada Gold Medal Student Award, NSERC Canada Graduate Scholarship and Vanier Canada Graduate Scholarship.
Breanna’s work at PBS Biotech focuses on designing robust and effective scale-up protocols for the expansion of high quality iPSCs to overcome bioprocess bottlenecks and assist customers in bringing life changing cell and gene therapies to market.
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