3D cancer model

Tissue-engineered 3D cancer models have grown in popularity with recent advances in cancer research. 3D models are more biomimetic than 2D cell monolayers cultured on tissue culture plastic. Examples are spheroids, tumorspheres, organoids, PDX and lately, tumoroids, which differ by origin (cell line or patient-derived), size, the complexity of cellular architecture and tumor environment.

Discover more about how to develop 3D cancer models and other complex organoids in this resource >>>

Cancer stem cell selection​

CSC therapy – towards new opportunities​

Cancer stem cells (CSCs) are a small subset of tumor cells that are able to evade anti-tumor defense mechanisms and suppress anti-tumor immune responses by releasing soluble immunosuppressive factors, such as anti-inflammatory cytokines. CSCs appear to have multiple strategies to escape immunological destruction, including the acquisition of genetic and non-genetic alterations that reduce immune recognition, enhance tolerance against soluble factors and promote the establishment of an immunosuppressive tumor microenvironment.

Discover how this toolbox allows for pure CSC isolation and expansion for advanced CSC therapy development >>>

From biopsy to assay development​

Struggling to get high-quality biopsies? Isolating patient-derived cancer cells from different types of biopsies is the golden standard for cancer research. However, obtaining regular surgical biopsies from patients is challenging. Discover tips for maximizing biopsy use for assay development in this toolkit >>>

Making the best use of your patient-derived xenograft mouse​

Xenograft models are extensively used to provide information about the safety and efficacy in drug development, especially for targeted cancer therapies. Patient-derived xenograft mice are the preferred mouse tumor models, but cell line-derived xenografts and nude rat xenografts are also used. Nevertheless, too many drugs still fail at the clinic that seemed promising in such models. More predictive data are needed to increase optimal translation to the clinic.

Explore methods for leveraging your patient-derived xenograft mouse project for better decision-making and translation to the clinic >>>

Extracellular vesicles in cancer therapy

Extracellular vesicles, including exosomes, play a crucial role in tumor biology. Alongside modulating the tumor microenvironment and contributing to the malignant cascade (angiogenesis, the host immune system response and metastasis), extracellular vesicles are involved in chemoresistance. Docetaxel in prostate cancer, for example, may transport mRNA encoding drug-resistant proteins or carry miRNA to recipient cells and trigger the expression of specific target genes.

Learn more about the application of extracellular vesicles for cancer therapy >>>

Immuno-oncology​

Immuno-oncology is a unique combination of oncology and immunology. By activating or amplifying the body’s natural defenses, immuno-oncology aims to boost the immune system to fight cancer better. Targeted antibodies or immunomodulators are well-known immunotherapies; adoptive cell therapy using CAR-T or TCR cells is particularly important, representing a significant breakthrough in cancer therapy with the recent approval of several CAR-T therapies.

Discover more about how relevant, reliable and scalable 3D in vitro assays are needed to enhance the development of immuno-oncology therapies >>>

Tumor microenvironment​

Cellular interactions in the tumor microenvironment significantly influence cancer progression and drug response. The efficacy of immunotherapies has triggered increased interest in the tumor immune microenvironment. Therefore, robust experimental systems are needed to enable researchers to model patient-specific interactions between tumor cells and immune cells.

Set up a complex organoid model combining cancer and non-cancer cells with the help of this toolbox >>>

Primary cells in respiratory research – webinar​

Learn about the benefits of primary cells as model systems in respiratory research and how a 3D model at the Air-Liquid Interface can be set up and used. Watch the webinar.

​Macrophage subtypes: polarization, activation and plasticity​

Understand the importance of activation and polarization of macrophages. Explore macrophage biology.​

Cells in action: differentiation of human mesenchymal stem cells into neurons​

Follow live cell differentiation and observe MSC-to-neuron transitions in real time. See live imaging.

​HLA-typed primary cells: the advantages of working with HLA in drug development research​

Understand the benefits of working with HLA-typed cells in your pre-clinical research. Explore HLA.

How to achieve a reliable Air-Liquid Interface (ALI) culture​

Learn how to use our Air-Liquid Interface Culture system for respiratory research in an environment that replicates in vivo. Watch the video.

3D cancer model

Tissue-engineered 3D cancer models have grown in popularity with recent advances in cancer research. 3D models are more biomimetic than 2D cell monolayers cultured on tissue culture plastic. Examples are spheroids, tumorspheres, organoids, PDX and lately, tumoroids, which differ by origin (cell line or patient-derived), size, the complexity of cellular architecture and tumor environment.

Discover more about how to develop 3D cancer models and other complex organoids in this resource >>>

Cancer stem cell selection​

CSC therapy – towards new opportunities​

Cancer stem cells (CSCs) are a small subset of tumor cells that are able to evade anti-tumor defense mechanisms and suppress anti-tumor immune responses by releasing soluble immunosuppressive factors, such as anti-inflammatory cytokines. CSCs appear to have multiple strategies to escape immunological destruction, including the acquisition of genetic and non-genetic alterations that reduce immune recognition, enhance tolerance against soluble factors and promote the establishment of an immunosuppressive tumor microenvironment.

Discover how this toolbox allows for pure CSC isolation and expansion for advanced CSC therapy development >>>

From biopsy to assay development​

Struggling to get high-quality biopsies? Isolating patient-derived cancer cells from different types of biopsies is the golden standard for cancer research. However, obtaining regular surgical biopsies from patients is challenging. Discover tips for maximizing biopsy use for assay development in this toolkit >>>

Making the best use of your patient-derived xenograft mouse​

Xenograft models are extensively used to provide information about the safety and efficacy in drug development, especially for targeted cancer therapies. Patient-derived xenograft mice are the preferred mouse tumor models, but cell line-derived xenografts and nude rat xenografts are also used. Nevertheless, too many drugs still fail at the clinic that seemed promising in such models. More predictive data are needed to increase optimal translation to the clinic.

Explore methods for leveraging your patient-derived xenograft mouse project for better decision-making and translation to the clinic >>>

Extracellular vesicles in cancer therapy

Extracellular vesicles, including exosomes, play a crucial role in tumor biology. Alongside modulating the tumor microenvironment and contributing to the malignant cascade (angiogenesis, the host immune system response and metastasis), extracellular vesicles are involved in chemoresistance. Docetaxel in prostate cancer, for example, may transport mRNA encoding drug-resistant proteins or carry miRNA to recipient cells and trigger the expression of specific target genes.

Learn more about the application of extracellular vesicles for cancer therapy >>>

Immuno-oncology​

Immuno-oncology is a unique combination of oncology and immunology. By activating or amplifying the body’s natural defenses, immuno-oncology aims to boost the immune system to fight cancer better. Targeted antibodies or immunomodulators are well-known immunotherapies; adoptive cell therapy using CAR-T or TCR cells is particularly important, representing a significant breakthrough in cancer therapy with the recent approval of several CAR-T therapies.

Discover more about how relevant, reliable and scalable 3D in vitro assays are needed to enhance the development of immuno-oncology therapies >>>

Tumor microenvironment​

Cellular interactions in the tumor microenvironment significantly influence cancer progression and drug response. The efficacy of immunotherapies has triggered increased interest in the tumor immune microenvironment. Therefore, robust experimental systems are needed to enable researchers to model patient-specific interactions between tumor cells and immune cells.

Set up a complex organoid model combining cancer and non-cancer cells with the help of this toolbox >>>

Cancer organoid co-culture protocol​

3D human airway model with air-liquid interface culture​

This straightforward protocol will walk you through the initial 2D phases of culture and expansion of primary human nasal and tracheal epithelial cells. You’ll then move to the 3D phases of proliferation and differentiation using our Air-Liquid Interface Culture System.

Start your Air-Liquid Interface culture now >>>

Patient-derived xenograft mouse​

Cancer stem cells in tumor biology​

Mesenchymal Stem Cells: Challenges and chances in cancer therapy​

As the potential for using mesenchymal stem cells in cancer therapy grows, understanding the biology behind them can help inform and inspire innovative cancer therapies. Read this blog post and find out how using high-quality media kits will increase the efficiency and usability of these highly demanded cells >>>

Engineering a beating heart: The promise of cardiac spheroids ​

The HLA complex​

Discover the role of the Human Leukocyte Antigen Complex in the immune system and learn about the relevance of HLA to cancer immunotherapy in this White Paper >>>​

Human Leukocyte Antigen​

Discover the importance of HLA for the development of cell therapies in this White Paper, including its restrictions for finding and binding, its role in disease, such as cancer, and new therapeutic options for harnessing HLA for cancer immunotherapy.

MSCs in cell and gene therapy​

How to start your culture: Thawing primary cells ​

Proper cell thawing is essential for the successful initiation of reliable cell cultures. In this video, we will show you how to thaw cells and prepare human primary cells for your research. Watch the video >>>​

How to achieve a reliable Air-Liquid Interface culture ​