Organoids are 3D miniaturized in vitro tissue constructs that are derived from stem cells to show realistic micro-anatomy. They are capable of self-renewal, self-organization and can exhibit similar functionality to the tissue of origin. Although the current methods for generating organoids are still evolving, these models are still providing exciting and more accurate systems that are advancing our understanding of basic organ biology and tissue regeneration.
This eBook provides an exciting resource for key publications, protocols, reagents and troubleshooting recommendations for organoid cell culture. Highlights from this eBook include publications that have used organoids to investigate questions about development, disease, or drug safety; recipes and protocols for how to culture different types of organoids; and links to the key reagents that are needed to ensure robust and reproducible organoid growth.
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