eBook: electroporation
Download peer-reviewed content and expert insights in our latest eBook.
Electroporation is a powerful transfection tool that utilizes an electrical pulse to transiently increase the permeability of the cell membrane, allowing nucleic acids or proteins to enter the cells. However, many cell types, such as primary T cells, natural killer cells, or hematopoeitic stem cells, cannot be efficiently transfected using traditional electroporation methods, presenting significant challenges to cell therapy development and manufacture.
This eBook explores the applications of electroporation and investigates how advanced electroporation technologies, such as nucleofection, can overcome challenges when working with difficult-to-transfect cell types.
Contents:
- [INTERVIEW] Beyond the barrier: an interview about non-viral transfection
- [REVIEW] Mesenchymal stem cells modifications for enhanced bone targeting and bone regeneration
- [POSTER] Inducible Cas9 T cells: An innovative platform for allogeneic CAR-T cell generation
- [RESEARCH ARTICLE] Efficient transient genetic labeling of human CD34+ progenitor cells for in vivo application
- [POSTER] Efficient transfection and sustained long term functionality of primary human hepatocytes
- [REVIEW] CRISPR-mediated modification of DNA methylation pattern in the new era of cancer therapy
- [BENCHMARK] Evaluation protocol for CRISPR/Cas9-mediated CD19 knockout GM24385 cells by flow cytometry and Sanger sequencing
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